Figure 4.

Characterization of pagN mutant strain ML6. (A) Colonies of strains SL1344 and ML6 were boiled and used as a template in a PCR reaction with primers PagNF2 and PagNR2 to amplify the pagN ORF. MW indicates molecular size markers. Lane 1 ML6, lane 2 SL1344. (B) Sarkosyl-extracted outer membrane proteins were electrophoresed through a 15% gel. Proteins were transferred to PVDF membrane and probed with an anti-PagN antibody. The position of PagN is arrowed. When expressed from a multicopy plasmid PagN was found to migrate as a doublet in common with the Hek protein. Lane 1, SL1344; Lane 2, ML6; Lane 3, ML6 with pBR322; Lane 4, ML6 with pML10.