|
S. Typhimurium mutants that exhibited different type 1 fimbrial phenotypes than the parental strain |
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| Category |
Mutant |
Phenotype |
Genea |
Function |
| (agar, broth) |
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|
|
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| Fimbrial biosynthesis and regulation |
K2 |
-, - |
fimZ |
Positive regulator of type 1 fimbriae major subunit gene fimA |
| K14 |
-, - |
fimY |
Positive regulator of type 1 fimbriae major subunit gene fimA |
|
| K40 |
-, - |
fimA |
Major subunit of type 1 fimbriae |
|
| K66 |
-, - |
fimD |
Fimbrial usher protein of FimA |
|
| K75 |
-, - |
fimC |
Fimbrial chaperone protein of FimA |
|
| K44 |
-, - |
fimH |
Adhesin of type 1 fimbriae |
|
| K70 |
+, + |
lpfB |
Fimbrial chaperone protein of long polar fimbriae |
|
| K61 |
+, + |
pefI |
Regulator of plasmid-encoded fimbriae |
|
| K23 |
+, + |
stbC |
Fimbrial usher protein of Stb fimbriae |
|
| Putative cytoplasmic protein |
K1 |
+, + |
STM1078 |
Putative cytoplasmic protein |
| K28 |
+, + |
STM4529 |
Putative cytoplasmic protein |
|
| Global regulator |
K46 |
-, - |
lrp |
Leucine-responsive regulatory protein |
| K15 |
-, - |
hupA |
Histone-like DNA binding protein |
|
| Membrane associated protein |
K17, |
+, + |
yhjV |
Putative transporter protein |
| K39 |
-, - |
STM1128 |
Putative sodium/glucose cotransporter |
|
| K45 |
-, - |
yihO |
Putative glycoside-pentoside-hexuronide (GPH) family transport protein |
|
| K50 |
+, + |
STM2532 |
Putative inner membrane lipoprotein |
|
| K52 |
+, + |
STM2486 |
Putative inner membrane protein |
|
| K54 |
-, - |
yjiH |
Putative inner membrane protein |
|
| K72 |
+, + |
mgtB |
Mg2+ transporter protein |
|
| K30 |
+, + |
narK |
Nitrate/nitrite transporter protein |
|
| Cell envelope associated protein |
K60 |
+, + |
ytfB |
Putative cell envelope opacity-associated protein A |
| Periplasmic protein |
K69 |
+, + |
ydbH |
Putative periplasmic protein |
| Ribosome modulation factor |
K56 |
-, - |
rmf |
Ribosome modulation factor |
| Type III secretion system |
K55 |
-, - |
ssaB |
Effector protein of type III secretion system |
| K59 |
+, + |
invB |
Chaperone protein for SopE/SopE2 |
|
| K63 |
-, - |
ssaV |
Apparatus protein for intracellular trafficking and secretion |
|
| Prophage-derived protein |
K11 |
+, + |
STM4200 |
Putative phage tail protein H |
| K33 |
+, + |
sseI |
Gifsy-2 prophage putative type III secreted effector protein |
|
| K19 |
-, - |
STM1041 |
Gifsy-2 prophage probable minor tail protein |
|
| Sensor protein |
K51 |
+, + |
ssrA |
Sensor component of type III secretion system |
| K58 |
+, + |
yehU |
Sensor component |
|
| Transcription termination factor |
K73 |
-, - |
nusA |
Transcription termination factor |
| Enzymes |
K3 |
-, - |
cafA |
Endoribonuclease |
| K5 |
+, + |
res |
Subunit of type III restriction-modification enzyme |
|
| K8 |
+, + |
STM4467 |
Putative arginine deiminase |
|
| K16 |
+, + |
STM1627 |
Alcohol dehydrogenase class III |
|
| K31 |
+, + |
STM2446 |
Putative iron-dependent peroxidase |
|
| K41 |
-, - |
nuoE |
NADH dehydrogenase I chain E |
|
| K37 |
+, + |
yafA |
Putative hydrolase |
|
| K43 |
-, - |
miaA |
Isopentenylpyrophosphate transferase |
|
| K38 |
-, - |
prC |
C-terminal protease for penicillin binding protein |
|
| K47 |
-, - |
atpH |
Membrane-bound ATP synthase |
|
| K48 |
+, + |
ubiB |
NAD(P)H-flavin reductase |
|
| K49 |
-, - |
ydiF |
Putative acetyl-CoA: acetoacetyl-CoA transferase |
|
| K53 |
+, + |
tdK |
Thymidine kinase |
|
| K65 |
+, + |
pps |
Phosphoenolpyruvate synthase |
|
| K57 |
+, + |
ybjX |
Putative enzyme with unknown function |
|
| K67 |
+, + |
pncA |
Nicotinamidase/pyrazinamidase |
|
| K68 |
+, + |
rfaB |
Lipopolysaccharide 1,6-galactosyltransferase |
|
| K71 |
+, + |
xapB |
Xanthosine permease |
|
| K64 |
+, + |
STM1940 |
Putative cell wall-associated hydrolase |
|
| K74 |
+, + |
cbiA |
Cobyrinic acid a, c-diamide synthase |
|
| K42 |
-, - |
aroA |
3-enolpyruvylshikimate-5-phosphate synthetase |
|
|
a: The gene that was disrupted by the transposon insertion is listed. The annotation of S. Typhimurium LT2 genome is used if the transposon insertion was present in a gene which is uncharacterized and unnamed. | ||||
Chuang et al. BMC Microbiology 2008 8:126 doi:10.1186/1471-2180-8-126 |
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