Figure 2.

A representative Southern blot demonstrating hybridization of the genomic DNA cleaved with EcoRI with a DIG-labeled 1.3-kb kanamycin-resistance cassette DNA probe. Lane 1: pUC4K plasmid DNA (positive control); lane 2: S. Typhimurium LB5010 (negative control); lanes 3 to 10: K1 to K8 strains, respectively.