ureB gene detection of H. pylori by real-time PCR using Taqman probe. Control positive are a fragment of 97 bp from ureB gene of H. pylori 26695 (ATCC 700392) cloned in pPCR-TOPO 2.1 (Invitrogen). High and low positive control equivalent to approximately 6 × 106 and 60 copies of the ureB gene, respectively.
Castillo-Rojas et al. BMC Microbiology 2008 8:119 doi:10.1186/1471-2180-8-119