Figure 3.

Quantification of DENV-2 RNA and infectious virus in midguts revealed significant differences. Midguts (n = 15) from orally infected Chetumal mosquitoes were assayed to quantify viral RNA by Q-PCR and infectious particles by end point titrations at different time points. A) Viral RNA decreased slightly between 7 and 14 dpi and then remained stable, * P < 0.05. B) Viral titers declined significantly at 14 and 21 dpi, *** P < 0.0001.