Table 2

Influence of mismatch position on weighted mismatch calculation

5' – 3' Position


1

2

3

4

N

3

2

1

0.3

0.6

1.0

1.2

1.2

1.1

0.8

0.3


Weights for mismatches related to their position in the sequence. A single mismatch at the first position of the probe is weighted with 0.3 whereas mismatches at central positions of the probes were weighted highest with 1.2. These values were experimentally determined.

Wiesinger-Mayr et al. BMC Microbiology 2007 7:78   doi:10.1186/1471-2180-7-78

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