VOPBA and DENV2 affinity chromatography of the virus dengue receptors. Proteins from Aedes aegypti MGs were extracted with 0.05% Triton X-100, separated by 10% SDS-PAGE and blotted on to a PVDF membrane. The putative receptors were revealed after incubation with DEN2 virus and peroxidase-labeled goat anti-mouse antibody (panel A, lane 1) as described in the methods section. Receptors were purified from C6/36 cell membranes (panel A, lane 3) or MG (fractions 1 and 2, panel B) by DENV2 affinity chromatography using a DEN2-Sepharose™ 4B column as described in the methods section. The apparent molecular weights of these proteins are shown on the right side of panel A and on the left side of panel B. Proteins separated by SDS-PAGE were silver stained. The protein pattern of total C6/36 membranes is shown in panel A lane 2. Molecular weight markers are shown on the left side in panel A and on the right side in panel B.
Mercado-Curiel et al. BMC Microbiology 2006 6:85 doi:10.1186/1471-2180-6-85