Table 4

Determination and characterization of erythromycin and clindamycin MICs and molecular characterization of Brucella eryR mutants.

Brucella eryR strains

ERY

ERY + PAβN

CL

CL + PAβN

Loci

Mutation


B. suis

WT

1.5

0.75

8

4

NA

a

6

6

12

12

-

b

6

6

12

12

ND

c

4

6

8

8

-

d

6

6

12

12

ND

e

6

6

8

8

ND


B. canis

WT

0.5

0.094

8

0.5

NA

a

2

0.32

8

1.0

-

b

2

0.047

6

<0.016

-

c

3

0.047

8

0.38

-

d

3

0.047

4

0.125

-

e

2

0.19

8

3

-


B. neotomae

WT

0.75

0.047

3

0.75

NA

a

4

0.125

8

0.75

-

b

3

0.125

6

2

ND

c

2

0.064

6

1.5

-

d

6

0.25

6

1.0

L22

6 bp indel, β-loop

e

2

0.38

4

2

-


Propoise

WT

1.5

0.25

16

3

NA

a

16

0.75

4

3

L4

G209A; Gly70Asp

b

>256

>256

>256

>256

23S

A2058G (Ec)

c

192

1.5

4

3

L4

Δ18aa (54–71)

d

48

0.38

6

3

L4

G209A; Gly70Asp

e

128

1.5

4

3

L4

Δ30aa (54–81)


Seal

WT

6

1.5

>256

48

NA

a

24

12

96

96

-

b

32

24

>256

>256

-

c

>256

>256

16

16

L4

C217A; Arg73Ser

d

128

16

>256

>256

-

e

128

24

>256

>256

ND


Dolphin

WT

3

0.38

2

0.75

NA

a

>256

128

1.0

1.5

L4

G209A; Gly70Asp

b

>256

6

1.5

1.0

L4

C217T; Arg73Cys

c

>256

192

1.5

1.5

L4

G209A; Gly70Asp

d

>256

8

2.0

1.0

L4

C217T; Arg73Cys

e

>256

8

1.5

1.0

ND


Mutants examined were isolates of B. suis biovar 1, B. canis, B. neotomae, and marine porpoise, seal and dolphin strains as per Table 1; WT, wild type; Ery, erythromycin; CL, clindamycin, PAβN, efflux inhibitor L-phenylalanine-L-arginine-β-naphthylamide; -, no mutation identified in any of three ribosomal loci examined; NA, not applicable; ND, sequence not determined.

Halling and Jensen BMC Microbiology 2006 6:84   doi:10.1186/1471-2180-6-84

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