Table 2

Oligonucleotide primers for cDNA synthesis and PCR.

Primer set

Primer

Nucleotide sequence (5' to 3')

Nucleotide position *

Polarity


EV1

EV1F

TAA AAC AGC YKK KGG KTK KYA CC

2–23

sense

EV1R

TGM ACR TGR ATG CAR AAC C

1268–1286

antisense

EV2

EV2F

CCD GAT GTR YTR ACW GAA ACC

1200–1221

sense

EV2R

ATC ACA TCT GCC ACY CTR TCY

2443–2463

antisense

EV3

EV3F

GGR GCR CCY AAY ACW GCY TAY ATW WTR GC

2333–2361

sense

EV3R

CYT GRG CRG TRG TWG AHG AHA CKA RB

3451–3476

antisense

EV4

EV4F

CAR GTY TCH GTB CCR TTY ATG TCA CC

3005–3030

sense

EV4R

GCT GTY TTB GMY TTR AYC CAV GC

4022–4044

antisense

EV5

EV5F

YAA CTC WMD SAG AAA RCA CTA YCC

3526–3559

sense

EV5R

GWS GAK GCA ATS ACA AAC TTD GAK G

4719–4743

antisense

EV6

EV6F

CCW GAY CAY TTT GAY GGR TAY MAR C

4559–4583

sense

EV6R

AHC CRT ACT GVV CVA CRT CHY C

5738–5759

antisense

EV7

EV7F

GTT GAG CGH CAC YTS AAY AGA GY

5216–5238

sense

EV7R

GYA RRT CYA ACC CAT ACT TRT CC

6373–6395

antisense

EV8

EV8F

CAT YAA GAA RAR RGA CAT YYT BG

6310–6332

sense

EV8R

TTG CTA TTC TGG TTA TAA CAA ATT TAC CC

7385–7413#

antisense


* The nucleotide position relative to the EV-71 MS87 strain

# Include 2 positions in poly-A tail

R = G/A Y = T/C M = A/C K = G/T S = G/C W = A/T

H = not G B = not A V = not T D = not C

Yoke-Fun and AbuBakar BMC Microbiology 2006 6:74   doi:10.1186/1471-2180-6-74

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