A: Schematic representation of the 230 bp DNA probes used for mobility shift assays. The positions of the intact LlaJI recognition sequences are indicated. B: Mobility shift assay with specific and non-specific probes (as indicated) which were incubated with increasing concentrations of purified R1.LlaJI. Final concentrations of protein are specified above each lane. C: Mobility shift assay with probe J which was incubated with increasing concentrations of purified R1.LlaJI (as indicated above each lane). The presence (+) or absence (-) of 5 mM GTP/ATP is as specified.
O'Driscoll et al. BMC Microbiology 2006 6:40 doi:10.1186/1471-2180-6-40