Table 3

Influence of SYBR Green I concentration on the melting temperature of the PCR product specific for F. graminearum. 50 pg of Fg3 DNA were used as a template for PCR with the primer pair Fg16N F/R in addition to different SYBR Green I concentrations and 3 mM MgCl2. The other parameters were set as described in the Methods.

SYBR Green I concentration in the amplification mix

Melting temperature of the 280 bp PCR product


0.1×

84.5°C

0.2×

85.0°C

0.3×

85.5°C

0.4×

86.0°C

0.7×

86.5°C


Brandfass and Karlovsky BMC Microbiology 2006 6:4   doi:10.1186/1471-2180-6-4

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