Additional File 1.
DuplexPCRFusarium. The pre-formatted Excel spreadsheet facilitates automatic data processing and reporting. In short, data concerning the two largest maxima identified on the first derivative of each melting curve are processed as follows: the size of the peaks are checked against pre-set minimal values, the melting temperatures are compared with values expected for both species-specific PCR products, and the final results are presented in a tabular report.
Format: XLS Size: 156KB Download file
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Brandfass and Karlovsky BMC Microbiology 2006 6:4 doi:10.1186/1471-2180-6-4