Section of the interpretation sheet corresponding to 4 wells. Each result consists of a well position, sample identifier, CT value, melting temperatures, areas under the melting curve peaks, and fractions of these areas in relation to the total area under the melting curve. The melting data are calculated for the two products with the highest melting temperatures only. Fields are automatically highlighted in a species-specific manner (green: F. culmorum, yellow: F. graminearum) and a warning is issued by a red coloring of the fields when the peak size does not reach the preset absolute and relative minimal values. In addition, if there is an unexpected third melting-curve maximum at temperatures higher than 80°C (indicating the presence of additional PCR products from F. culmorum or F. graminearum), the corresponding well will be highlighted in orange to indicate that the user should check the raw data.
Brandfass and Karlovsky BMC Microbiology 2006 6:4 doi:10.1186/1471-2180-6-4