SYBR green 1 dye PCR assay to determine the frequency of prophage excision. The amplification profiles in triplicates (marked 1–6) with varying concentrations of the template DNA (34F2 genomic DNA) and primer pairs are shown. The corresponding template concentrations and the fragments amplified are as follows: 1-1 ng/glp, 2-100 pg/glp, 3–10 pg/glp, 4-1 pg/glp, 6-100 fg/glp, 5-100 ng/Ba03mtF and R. The standard curve generated from the glp set is shown on the right panel.
Sozhamannan et al. BMC Microbiology 2006 6:34 doi:10.1186/1471-2180-6-34