Multiplex PCR assay for confirmation of B. anthracis DNA. Multiplex PCR analysis of B. cereus and B. anthracis strains using primers (ORF01192 F-R, ORF02190 F-R, ORF03655 F-R and lambda Ba02 #7 For-Rev). K-1 kb plus ladder (Invitrogen, Inc); E-E-gel low-range marker (Invitrogen, Inc). Lanes 1–9 are B. cereus strains; S74, S363, SPS2, F3080B, F3942/87, F4801/72, m1292, F4801 and S710 respectively. Lane 10-B. anthracis strain 34F2, Lane 11-B. cereus G9241 and Lane 12-reaction control. Panel A reactions were carried out with the multiplex primer set and panel B reactions were carried out with a primer pair for a house-keeping gene gmk in addition to the multiplex primer set.
Sozhamannan et al. BMC Microbiology 2006 6:34 doi:10.1186/1471-2180-6-34