Table 1

Study characteristics and methodological quality of included studies

Study characteristic

Frequency [N = 84 studies]


Study design

Cross sectional

60 (71%)

Case-control

24 (29%)

Blinded assessment of NAA test and reference standard results

Double or single blinded

29 (34%)

Unblinded or Unknown

55 (66%)

Target sequence for amplification

IS6110

54 (64%)

Other target sequences

30 (36%)

Use of chemical-based DNA extraction method

Any chemical method

55 (66%)

Physical methods

29 (34%)

Amplification technique

Nested or semi-nested PCR

16 (19%)

Regular or multi-plex PCR

68 (81%)

Detection of amplified products

Probe-based method

49 (58%)

Gel-UV method

35 (42%)


Flores et al. BMC Microbiology 2005 5:55   doi:10.1186/1471-2180-5-55

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