Table 3

Susceptibility of Escherichia coli JM109 to metal cations with 2 or 24 h of exposure in minimal (MSVG) medium

Periodic group

Metal ion

Exposure time (h)

MIC (mM)

MBC (mM)

MBEC (mM)

Fold Tolerance1


7B

Mn2+

2

33 ± 9

> 149

> 149

na

24

93 ± 37

84 ± 47

0.90

8B

Co2+

2

≤ 0.27

26 ± 10

> 139

≥ 11

24

1.0 ± 3

1.0 ± 3

1.0

Ni2+

2

≤ 0.27

> 139

> 139

na

24

0.48 ± 0.13

0.54 ± 0.39

1.1

1B

Cu2+

2

≤ 0.25

≤ 0.25

≤ 0.25

na

24

≤ 0.25

≤ 0.25

na

Ag+

2

≤ 0.04

≤ 0.04

0.59 ± 0

≥ 15

24

≤ 0.04

≤ 0.04

1.0

2B

Zn2+

2

≤ 0.25

11 ± 6

> 125

≥ 23

24

0.55 ± 0.31

0.49 ± 0.35

0.89

Cd2+

2

≤ 0.14

5.1 ± 2.9

> 73

≥ 29

24

0.49 ± 0.14

1.4 ± 1.1

2.9

Hg2+

2

≤ 0.02

≤ 0.02

0.05 ± 0.02

≥ 2.5

24

≤ 0.02

≤ 0.02

1.0

3A

Al3+

2

nd

42 ± 40

> 304

≥ 14

24

1.5 ± 0.6

4.0 ± 1.4

2.7

4A

Sn2+

2

2.2 ± 0

8.6 ± 0

8.6 ± 0

1.0

24

4.3 ± 0

7.5 ± 2.2

1.7

Pb2+

2

nd

20 ± 0

40 ± 0

2.0

24

4.9 ± 0

1.2 ± 0

0.25


na indicates a measurement that is not applicable

bold indicates the fold tolerance at 24 h of exposure

1the fold tolerance, given the sensitivity of the assay on a log2 scale, is equal to the ratio of the means of MBEC:MBC100

Harrison et al. BMC Microbiology 2005 5:53   doi:10.1186/1471-2180-5-53

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