Table 3 

Reproducibility of the LightCyclerPCR 

Copy number 
No. of PCR runs where fluorescence gave positive signal 
CP 
SD 
CV % of CP 


10^{5} 
10 / 10 
25.4 
1.7 
6.7 
10^{4} 
10 / 10 
28.7 
1.6 
5.5 
10^{3} 
10 / 10 
32.3 
1.6 
4.8 
10^{2} 
10 / 10 
35.8 
1.7 
4.8 
10^{1} 
10 / 10 
39.7 
2.1 
5.3 
5 copies 
10 / 10 
40.6 
2.3 
5.6 
1 copy 
6 / 10 
43.8 
1.7 
3.8 


CP = crossingpoints, mean value CV = coefficient of variation SD = standard deviation Statistical analysis of standard dilution series of standard PG21 DNA was performed. Ten different runs were analysed for crossingpoints values and for presence of fluorescence signal. Based on crossingpoints values the standard deviation of single concentration was calculated and coefficient of variation as a function: CV% = (SD / Average CP value for each concentration) × 100%. 

Baczynska et al. BMC Microbiology 2004 4:35 doi:10.1186/14712180435 