Table 3

Reproducibility of the LightCycler-PCR

Copy number

No. of PCR runs where fluorescence gave positive signal

CP

SD

CV % of CP


105

10 / 10

25.4

1.7

6.7

104

10 / 10

28.7

1.6

5.5

103

10 / 10

32.3

1.6

4.8

102

10 / 10

35.8

1.7

4.8

101

10 / 10

39.7

2.1

5.3

5 copies

10 / 10

40.6

2.3

5.6

1 copy

6 / 10

43.8

1.7

3.8


CP = crossing-points, mean value

CV = coefficient of variation

SD = standard deviation

Statistical analysis of standard dilution series of standard PG21 DNA was performed. Ten different runs were analysed for crossing-points values and for presence of fluorescence signal. Based on crossing-points values the standard deviation of single concentration was calculated and coefficient of variation as a function: CV% = (SD / Average CP value for each concentration) × 100%.

Baczynska et al. BMC Microbiology 2004 4:35   doi:10.1186/1471-2180-4-35

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