Sensitivity of LightCycler PCR in standard dilution series. (a) The LightCycler PCR run with M. hominis PG21 DNA and fluorescence probes was done. The fluorescence signal of 10-fold dilution series from 105 to 5 and 1 copy is shown. No reaction was noted in the negative control (0 copies). (b) Standard curve was generated from the threshold cycles (Ct) also known as crossing points (Cp) of the M. hominis PG21 standard dilution series by the LightCycler software.
Baczynska et al. BMC Microbiology 2004 4:35 doi:10.1186/1471-2180-4-35