Agarose gel of RT-PCR products. Reverse-transcriptase PCR (RT-PCR) of total RNA isolated from wild-type strain Rd after 18 hours of contact with either HBMEC (human endothelial cell) or NCI-H292 (human epithelial cell) monolayers. Lane A: Molecular weight marker (Promega 1 Kb ladder); lane B: RNA from Rd on HBMEC monolayers; lane C: Negative control for lane B (no reverse transcriptase added prior to PCR); lane D: RNA from Rd on NCI-H292 monolayers; lane E: Negative control for lane D (no reverse transcriptase added prior to PCR).
Daines et al. BMC Microbiology 2004 4:30 doi:10.1186/1471-2180-4-30