Table 1

Optimal m.o.i. values using RRV C6/36 stock virus with respective HA titre and hours of incubation in C6/36, PS-EK, Vero and BHK-21 C13 cell lines

Cell Line

m.o.i. (PFU/cell)

Maximum HA Titre at pH 5.8

Hours of Incubation


C6/36

1.5 × 10-4

1024

51

1.5 × 10-5

1024

51

PS-EK

1.1 × 10-1

1024

47

4.4 × 10-2

1024

47

2.2 × 10-2

1024

51

Vero

7.5 × 10-2

8192

47

3.0 × 10-2

8192

47

1.5 × 10-2

8192

47

1.5 × 10-3

8192

47

1.5 × 10-4

8192

47

BHK-21 C13

7.9 × 10-2

8192

51

3.2 × 10-2

8192

47

1.6 × 10-2

8192

47

1.6 × 10-3

8192

51


Pyke et al. BMC Microbiology 2004 4:3   doi:10.1186/1471-2180-4-3

Open Data