Table 1

msp2 hypervariable region primers to determine newly identified msp2 paralogs in low passage A. phagocytophilum genomic DNA.

msp2 transcript locus

Forward primer sequence (name)

Reverse primer sequence (name)

msp2 (pan-msp2)

5'-TGATGTTGTTACTGGACAGA-3' (MSP465f)

5'-CACCTAACCTTCATAAGAA-3' (MSP980r)

RW1

5'-GCGACGAGGAAGGACAGTGGT-3'

5'-AGTCTTCAACCCAGTCCCGCT-3'

RW17

5'-GTGACGAAGAATTACGACAGT-3'

5'-GCTAGAAGATCCGGTTCCTGT-3'

RW20

5'-GTGACGAAGAAGGGGACAAAC-3'

5'-GTTAGAGGCTCCGGTTCCTGT-3'

RW23

5'-ACAAAGCGGAAGGATGGTGAT-3'

5'-ATTCGATTAGACGTTTCACT-3'

Scorpio et al. BMC Microbiology 2004 4:1   doi:10.1186/1471-2180-4-1