Table 3

Publications with positive/negative results on direct detection by PCR of C. pneumoniae in atheroscle-rotic tissue

Authors

Year

PCR method

DNA purification

Samples collected in

PCR positive (%)

Wessely et al. [20]

2003

Semi-nested, 474-bp PstI fragment

phenol/chloroform

Germany

0/31 (0.0)

Nested, MOMP gene

Vainio et al. [21]

2002

Nested, MOMP gene

QIAmp Tissue kit

Norway

0/48 (0.0)

Ong et al. [22]

2001

Nested, MOMP gene

QiAmp Tissue Kit

Northern Ire

0/44 (0.0)

Meijer et al. [23]

2000

Single-step, 16S rRNA and MOMP genes

Easy-DNA Kit, modified

The Netherlands

0/13 (0.0)

Meijer et al. [24]

1999

Single-step, 16S rRNA and MOMP genes

Easy-DNA Kit, modified

The Netherlands

0/18 (0.0)

Palfrey et al. [15]

1999

Single-step, MOMP gene

Gene Clean Kit

England

0/8 (0.0)

Lindholt et al. [6]

1998

Nested, MOMP gene

phenol/chloroform

Denmark

0/124 (0.0)

Andreasen et al. [7]

1998

Multiplex, Chlamydia species

Chelex 100

Denmark

0/22 (0.0)

Daus et al. [25]

1998

Semi-nested, 474-bp PstI fragment

Proteinase K

Germany

1*/29 (3.4)

Paterson et al. [26]

1998

Nested, omp1 gene

Proteinase K

Australia

0/49 (0.0)

Weiss et al. [27]

1996

Single-step, 16S rRNA gene

Proteinase K

Brooklyn, USA

0/58 (0.0)

Cochrane et al. [28]

2002

Single-step, 474-bp PstI fragment

Qiamp Tissue Kit

Australia

15/29 (51.7)

Valassina et al. [29]

2001

Single-step, omp1, 16S rDNA, Hsp70 gene

Qiamp Tissue Kit

Italy

13/58 (58.0)

Gutierrez et al. [30]

2001

Semi-nested, 474-bp PstI fragment

High Pure PCR Template preparation Kit

Spain

48/85 (56.5)

Farsak et al. [31]

2000

Single-step, 16S rDNA

phenol/chloroform +CTAB

Turkey

12/46 (26)

Jantos et al. [32]

1999

Single-step, omp1, 16S rDNA

Proteinase K

Germany

4/50 (8.0)

Wong et al. [33]

1999

Nested, omp1

phenol/chloroform

England

26/68 (38)

Petersen et al. [34]

1998

Single-step, omp1

Qiamp Tissue Kit

Sweden

14/40 (35.0)

Ouchi et al. [35]

1998

Nested, 474-bp PstI fragment

phenol/chloroform

Japan

16/29 (55.2)

Maass et al. [36]

1997

Nested, 474-bp PstI fragment

phenol/chloroform +CTAB

Germany

9/61 (14.8)

Ong et al. [37]

1996

Nested, MOMP gene

phenol/chloroform

England

19/43 (44.2)

Blasi et al. [38]

1996

Nested, 16S rDNA

phenol/chloroform

Italy

26/51 (51.0)

Campbell et al. [39]

1995

Single-step, 474-bp PstI fragment

phenol/Chloro form or Qiamp

USA

12/38 (31.6)

Kuo et al. [40]

1993

Single-step, 474-bp PstI fragment, 16S rDNA

Phenol/chloroform

South Africa

12/30 (40.0)

*The single positive specimen was not positive on retesting

Mygind et al. BMC Microbiology 2003 3:19   doi:10.1186/1471-2180-3-19