|
Efficiency of transformation of deletions into TIGR4 |
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| Target gene |
homology upstream* |
homology downstream |
size of inserted marker |
size of deletion |
trans-forming DNA (ng/ml) |
trans-formants (CFU/ ml) |
selected trans-formant |
|
|
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| SP0071 – zmpC |
757 (including 27 bp of zmpC) |
807 (including 1 bp of zmpC) |
1184 (cat) |
5543 |
500 |
100 |
FP122 |
| SP0664 – zmpB |
715 (including 25 bp of zmpB) |
838 (from 7 bp after zmpB) |
894 (ermB) |
5628 |
1500 |
10 |
FP173 |
| SP1154 – iga |
801 (including 11 bp of iga) |
828 (including 27 bp of iga) |
850 (spe) |
6004 |
1500 |
120 |
FP174 |
|
* all sizes are expressed in base pairs. The total number of transformants obtained transforming 1 μg/ml were 5.2 × 102 CFU/ml using a chromosomal DNA carrying a point mutation conferring novobiocin resistance (positive control). | |||||||
Chiavolini et al. BMC Microbiology 2003 3:14 doi:10.1186/1471-2180-3-14 |
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