Table 3

Bacterial strains and plasmids used in this study.

Strain or plasmid

Genotype or description

Source or reference


Strains

Escherichia coli

TG1

K12 supE hsdΔ5 thi Δ (lac-proAB) F' [traD36 proA+ proB+ lacIqlacZΔM15]

Laboratory collection

XL1-Blue

K12 supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac- F' [proAB+ lacIqlacZΔM15 Tn10(tetR)]

Laboratory collection

Bacillus subtilis

168

trpC2

[31]

BSIP1143

trpC2 metI::spc

[32]

BSHP7010

trpC2 mtnS::spc

[6]

BFS1850

trpC2mtnK::lacZ

Functional analysis projecta[6]

BFS1851

trpC2mtnU::lacZ

Functional analysis projecta[6]

BFS1852

trpC2mtnX::lacZ

Functional analysis projecta

BFS1853

trpC2mtnZ::lacZ

Functional analysis projecta

BSHP7014

trpC2mtnW::lacZ amyE::(pxylmtnXYZ)

This work

BSHP7015

trpC2amyE::(pxylmtnXYZ)

This work

BSHP7016

trpC2mtnY::lacZ

This work

BSHP7020

trpC2mtnV::lacZ

This work

BSHP7064

trpC2mtnW::Tn10

This work

BSHP7065

trpC2mtnY::Tn10

This work

Plasmids

pIC333

mini-Tn10 delivery vector, SpcR, EryR

[27]

pJM783

cloning vector, CmR, AmpR

[25]

pX

cloning vector, CmR, AmpR, pxyl promoter, amyE locus integration

[24]

pHPP7011

pJM mtnV::lacZ

This work

pHPP7014

pJM mtnW::lacZ

This work

pHPP7014bis

pJM mtnW::lacZ (bla::spcb)

This work

pHPP7015

pX pxyl mtnXYZ

This work

pHPP7016

pJM mtnY::lacZ

This work


a. This strain has been constructed in the frame of the EC project for the functional characterization of the genome of B. subtilis in Europe. b.spc is the spectinomycin resistance gene from Staphylococcus aureus.

Sekowska and Danchin BMC Microbiology 2002 2:8   doi:10.1186/1471-2180-2-8

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