Figure 2.

Identification of the mtn region promoters by primer extension. A. Identification of the transcription start site of the mtnU operon. The size of the extended product is compared to a DNA-sequencing ladder of the mtnU promoter region. Primer extension and sequencing reaction were performed with the same primer. The +1 site is marked by an arrow. B. Identification of the transcription start site of the mtnV gene. The size of the extended product is compared to a DNA-sequencing ladder of the mtnV promoter region. Primer extension and sequencing reaction were performed with the same primer. The +1 site is marked by an arrow. C. Identification of the transcription start site of the mtnKS gene. The size of the extended product is compared to a DNA-sequencing ladder of the mtnKS promoter region. Primer extension and sequencing reaction were performed with the same primer. The +1 site is marked by an arrow. D. Identification of the transcription start site of the mtnWXYZ operon. The size of the extended product is compared to a DNA-sequencing ladder of the mtnWXYZ promoter region. Primer extension and sequencing reaction were performed with the same primer. Two +1 sites are marked by arrows. E. Sequences of the corresponding promoter regions. Promoter sites are in capital letters and underlined (-35 and -10 boxes), and the transcription start sites are indicated by broken arrows (+1).

Sekowska and Danchin BMC Microbiology 2002 2:8   doi:10.1186/1471-2180-2-8
Download authors' original image