Table 1

Library of ARDRA profiles (combination of restriction patterns) obtained for mycobacterial species.

Species

Genbank Number

HhaI

MboI

RsaI

BstUI

Reference strains useda


M. tuberculosis complex

X52917

1

1

1

1

ITG 8021, IPB 92/0805

M. conspicuum

X88922

1'

1

2

2

M. intracellulare

X52927

1'

2

2

3

ITG 5913, ITG 5917

M. gastri

X52919

1'

4

1

1

M. kansasii

M29575

1'

4

1

1

ITG 8201

M. bohemicum

AJ277283

1'

4

1

3

M. haemophilum

U06638

1'

4

1

3

ITG 3065

M. malmoense

AF152560

1'

4

1

3

ITG 940611

M. szulgai

X52926

1'

4

2

1

ITG 4981

M. scrofulaceum

X52924

1'

4

2

3

ITG 4988

M. xenopi

X52929

1'

4

3

2

ITG 4986

M. heckeshornense

AF174290

1'

4

3

4

M. marinum

AF251565

2

1

1

1

ITG 1728

M. asiaticum

M29556

2

1

2

5

ITG 8182

M. terrae

X52925

2

1

4

2

ITG 4922

M. ulcerans

Z13990

2

1

9

1

ITG 724, ITG 1837

M. avium

AF306455

2

2

2

3

ITG 4991, ITG 2666

M. botniense

AJ012756

2

4

3

2

M. terrae-like MCRO6b

X93032

2

6

4

7

M. nonchromogenicum

X52928

2

6

4

7

ITG 4980

M. phlei

M29566

3

1

6

2'

M. elephantis

AJ010747

3

1

8

3'

M. chelonae group IVc

AJ416940

3

3

5

2'

ITG 7701

M. abscessus group IIIc

AJ419970

3

3

6

2'

ITG 98-1296

M. chelonae group 1c

AJ419969

3

3

6

2'

ITG 95-0026

M. immunogenum

AJ011771

3

3

6

2'

ITG 98-1289

M. farcinogenes

AF055333

4

1

6

7

M. fortuitum

X52933

4

1

6

7

SLZ A046

M. senegalense

M29567

4

1

6

7

M. septicum

AF111809

4

1

6

7

ITG 4166

M. intermedium

X67847

5

4

6

1'

M. interjectum

AJ272088

5

4

6

2'

ITG 96-116

M. heidelbergense

X70960

5

4

8

2'

M. simiae

X52931

5

7

6

2'

ITG 4485

M. lentiflavum

X80769

5

7

6

2'

M. peregrinumd

X52921

7

1

6

9

ITG 99-2069, ATCC 14467

M. gordonae

M29563

8

4

2

5

ITG 7838

M. wolinskyi

Y12871

10

1

1

2'

M. speciesa

AF028712

10

1

1

2'

M. goodii

Y12872

10

1

2

2'

M. smegmatis

AJ131761

10

1

2

2'

ITG 4995

M. kubicae

AF133902

10

1

6

1'

M. tusciae

AF058299

10

1

6

2'

M. flavescens

X52932

10

1

6

2'

VUB A016

M. genavense

X60070

10

7

6

2'

ITG 97-102

M. triplex

U57632

10

7

6

2'

M. chelonae group IIc

AJ419968

13

3

6

6

ITG 96-0295

M. branderi

X82234

14

1

1

9

M. celatume

L08169

14

1

1

10

M. triviale

X88924

15

1

10

10'

M. aurum

X55595

16

1

6

8


a. ITG: Institute for Tropical Medicine, Antwerp, Belgium; IPB: Institute Pasteur du Brabant, Brussels, Belgium; SLZ: Streeklaboratorium Zeeland, Goes, the Netherlands; VUB: Free University of Brussels, Brussels, Belgium. b. Strains designated MCRO6 have been studied by Turenne et al.[22] and Torkko et al.[33]. c. Sequences determined in this study. Roman numbering according to Portaels et al.[16], who distinguished four groups within the M. abscessus/M. chelonae complex, based on the 16S–23S rRNA spacer region. d. The GenBank sequence (AF058712) of strain ATCC 14467, submitted to GenBank as M. peregrinum, did not cluster within the M. fortuitum complex (Figure 8) and was highly similar to sequence Y12871 (M. wolinskyi). Moreover, the ARDRA profile calculated from sequence AF058712 (10-1-1-2') did not correspond with the profile we obtained for strain ATCC 14467 (4-1-6-7). The sequence obtained in this study from strain ATCC 14467 (submitted as AJ422046) was identical to the M. fortuitum sequence X52933. Sequence AF058712 is indicated as M. species in the table. e. Received as M. xenopi.

De Baere et al. BMC Microbiology 2002 2:4   doi:10.1186/1471-2180-2-4

Open Data