Table 3 |
||||||
|
Summary of cPCR assay. |
||||||
|
cPCR type |
Primer used |
Annealing Tm (°C) |
Length of DNA fragment (bp) |
Specificity |
||
|
|
|
|||||
|
Taget |
Competitor |
Sequence type |
Phylogenetic group |
|||
|
|
||||||
|
AB |
U515f and AB1173r |
60 |
658 |
431 |
KU21, KU25 |
Acetobacterium |
|
AZ |
B341f and AZ665r |
55 |
324 |
437 |
KU34 |
Parts of Azoarcus and Zoogloea |
|
BC |
B341f and U533r |
55 |
170–210 |
336 |
All |
Bacteria |
|
DB |
B968f and DB1286r |
55 |
318 |
437 |
KB14 |
Part of Desulfobacula |
|
DT |
U515f and DT1047r |
60 |
526 |
340 |
KB13, KB32, KU26 |
Part of Desulfotomaculum |
|
DV |
DV387f and DV701r |
55 |
314 |
415 |
KB1, KB33, KB38 |
Desulfovibrio |
|
EP |
B341f and EP710r |
55 |
369 |
465 |
KB2, KB8 |
Cluster-1 bacteria |
|
|
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|
Watanabe et al. BMC Microbiology 2002 2:23 doi:10.1186/1471-2180-2-23 |
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