Table 4

Determination of PCR efficiency in 20 spiked clinical samples. S.E.: standard error, Eff.: Efficiency, std. standard samples. For each of 20 randomly selected negative samples spiked with C. pneumoniae DNA, the slope was determined as described and the efficiency calculated from the slope. The slope of each sample was compared to the slope of the standard from the same run with a t-test and the P-values are shown. Therefore, some of the samples are compared to the same standard.

Sample no.

Slope sample

S.E. sample

Eff. sample

Slope std.

S.E. std.

Eff. std.

P-value*


1

-3.064

0.215

2.12

-3.406

0.232

1.97

0.28

60

-3.190

0.273

2.06

0.55

14

-2.873

0.147

2.23

-3.266

0.264

2.02

0.19

31

-3.222

0.091

2.04

-3.623

0.340

1.89

0.25

32

-3.549

0.156

1.91

0.84

78

-3.328

0.153

2.00

0.43

84

-4.318

0.512

1.70

-3.321

0.180

2.00

0.066

90

-3.379

0.177

1.98

0.82

97

-3.503

0.291

1.93

-3.716

0.187

1.86

0.54

101

-3.266

0.085

2.02

0.028

103

-3.388

0.091

1.97

0.12

118

-3.398

0.189

1.97

-3.492

0.094

1.93

0.65

127

-3.495

0.145

1.93

0.99

131

-2.809

0.108

2.27

0.00

138

-3.545

0.183

1.91

-3.300

0.101

2.01

0.24

145

-3.396

0.134

1.97

0.28

150

-3.652

0.394

1.88

0.39

159

-3.491

0.133

1.93

-3.615

0.056

1.89

0.39

160

-2.135

0.313

2.94

0.00

171

-3.531

0.088

1.92

0.42


Mygind et al. BMC Microbiology 2002 2:17   doi:10.1186/1471-2180-2-17

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