Table 2

Reference samples analysed with the two PCR assays. * The unit of the numbers is copies/μl and the numbers are the mean of two measurements from two different runs. # The dilution series is serial and four-fold. Dilution no. 1 has the highest concentration and dilution no. 10 has the lowest concentration. Method 1 is the real-time PCR and method 2 is the conventional PCR.
PCR method
 1
2
Detection
 probe set*
 gel
Dilution no. #

1 (high)
 76.60
 +
2
 7.86
 +
3
 2.35
 +
4
 0.63
 +
5
 0.05
 +
6
 0.00
 -
7
 0.00
 -
8
 0.00
 -
9
 0.00
 -
10 (low)
 0.00
 -
11 (NK)
 0.00
 -

Mygind et al. BMC Microbiology 2002 2:17   doi:10.1186/1471-2180-2-17