Table 2

Reference samples analysed with the two PCR assays. * The unit of the numbers is copies/μl and the numbers are the mean of two measurements from two different runs. # The dilution series is serial and four-fold. Dilution no. 1 has the highest concentration and dilution no. 10 has the lowest concentration. Method 1 is the real-time PCR and method 2 is the conventional PCR.

PCR method

1

2

Detection

probe set*

gel

Dilution no. #


1 (high)

76.60

+

2

7.86

+

3

2.35

+

4

0.63

+

5

0.05

+

6

0.00

-

7

0.00

-

8

0.00

-

9

0.00

-

10 (low)

0.00

-

11 (NK)

0.00

-


Mygind et al. BMC Microbiology 2002 2:17   doi:10.1186/1471-2180-2-17

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