Resolution:
## Figure 2.
Effect of difference in efficiency between standard samples and samples. This figure illustrates the error in determination of concentration by real-time
PCR at cycle no. 30 when the efficiencies differ between standard samples and samples.
Number of cycles: n, Efficiency standard samples: E_{std}, efficiency reference samples: E_{rs}, Number of amplicons at cycle n: N_{n}, Number of amplicons at cycle zero: N_{0}. When the two equations are divided by eachother it is seen that there is an 23-fold
underestimation of the concentration when E_{std} = 2.0 and E_{rs} = 1.8. From this calculation it is also evident that at higher cycle number the error
in concentration determination is larger.
Mygind |