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Open Access Research article

The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation

Tamara Zimaro1, Ludivine Thomas2, Claudius Marondedze2, Germán G Sgro1, Cecilia G Garofalo1, Florencia A Ficarra1, Chris Gehring2, Jorgelina Ottado1 and Natalia Gottig1*

Author Affiliations

1 Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas (IBR-CONICET) and Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario. Ocampo y Esmeralda, Rosario 2000, Argentina

2 Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia

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BMC Microbiology 2014, 14:96  doi:10.1186/1471-2180-14-96

Published: 18 April 2014

Abstract

Background

Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein secretion system (T3SS). The role of T3SS in bacterial pathogenesis is well established but whether this system is involved in multicellular processes, such as bacterial biofilm formation has not been elucidated. Here, the phytopathogen Xanthomonas citri subsp. citri (X. citri) was used as a model to gain further insights about the role of the T3SS in biofilm formation.

Results

The capacity of biofilm formation of different X. citri T3SS mutants was compared to the wild type strain and it was observed that this secretion system was necessary for this process. Moreover, the T3SS mutants adhered proficiently to leaf surfaces but were impaired in leaf-associated growth. A proteomic study of biofilm cells showed that the lack of the T3SS causes changes in the expression of proteins involved in metabolic processes, energy generation, exopolysaccharide (EPS) production and bacterial motility as well as outer membrane proteins. Furthermore, EPS production and bacterial motility were also altered in the T3SS mutants.

Conclusions

Our results indicate a novel role for T3SS in X. citri in the modulation of biofilm formation. Since this process increases X. citri virulence, this study reveals new functions of T3SS in pathogenesis.

Keywords:
Xanthomonas citri subsp. citri; Biofilm; T3SS; Proteomics