Open Access Research article

Transcription of the three HMG-CoA reductase genes of Mucor circinelloides

Gábor Nagy, Anita Farkas, Árpád Csernetics, Ottó Bencsik, András Szekeres, Ildikó Nyilasi, Csaba Vágvölgyi and Tamás Papp*

Author Affiliations

Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, H-6726, Hungary

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BMC Microbiology 2014, 14:93  doi:10.1186/1471-2180-14-93

Published: 14 April 2014

Additional files

Additional file 1: Table S1:

Primers used in the present study. Table S2. Main features of the three HmgR proteins. Figure S1. Amino acid sequence of the three HMG-CoA reductases of Mucor circinelloides aligned to other known HmgR proteins. Figure S2. Relative transcript levels of the M. circinelloides hmgR genes during the cultivation period. Relative transcript level of hmgR1 at 96 hours was taken as 1. Figure S3. Relative transcript levels of the M. circinelloides hmgR genes at different cultivation temperatures. Relative transcript level of hmgR1 at 25°C was taken as 1. Figure S4. Relative transcript levels of the M. circinelloides hmgR genes at different salt concentrations. Relative transcript level of hmgR1 of the untreated control was taken as 1. Figure S5. Relative transcript levels of the hmgR genes of M. circinelloides growing on different carbon sources. Relative transcript level of hmgR1 on YNB with glucose was taken as 1. Hyphal morphology on the different carbon sources are showed on the light micrographs. Figure S6. Relative transcript levels of the M. circinelloides hmgR genes under aerobic and anaerobic growth conditions. Relative transcript level of hmgR1 when the fungus was grown under aerobic condition was taken as 1. Morphology of MS12 under aerobic and anaerobic conditions are showed on the light micrographs. Figure S7. Reverse transcription - PCR of the investigated genes. PCR conditions and primers were the same as in the qPCR experiments. Figure S8. Maps of the plasmids used in this study. Figure S9. PCR amplification of the transferred plasmids from the M. circinelloides transformants. Primers used in these experiments were designed to the terminus of the gpdP (Gpdp) and the first part of each hmgR gene. Sequences of the primers are shown in Table S1.

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