Table 4

The specific primers for PCR amplification
Name Primer sequence
fpsssbNcoI 5′ GGA GGA
    C
    CA TGG
GGA ACG GAA CGT TAA ATA AAG TCA TG 3′
fpsssbHindIII 5′ TTA
    AAG CTT
TTA AAA AGG CAA ATC ATT TTC TAC AG 3′
pcrssbNcoI 5′ TTA
    CC
    A TGG
GGC GCG GTG TTA ATA AAG TTA TCA TC 3′
pcrssbHindIII 5′ TTA
    AAG CTT
TCA GAA CGG AAT GTC ATC GTC 3′
ptossbNcoI 5′ GGA GGA
    CC
    A TGG
CAG GAA CAC TCA ATA AAG TTA TGC 3′
ptossbHindIII 5′ TTA
    AAG CTT
TTA AAA GGG TAG ATC ATC TTC CTC 3′
pprssbNcoI 5′ GGA GGA
    CC
    A TGG
CCA GTC GTG GTG TAA ATA AGG 3′
pprssbBglII 5′ TTA
    AGA TCT
CTA GAA TGG GAT ATC ATC ATC AAA ATC 3′
dpsssbNcoI 5′ TTA
    CC
    A TGG
GGA TAA ATA AGG CAA TTT TAA TTG GTA ATC TAG 3′
dpsssbHindIII 5′ TTA
    AAG CTT
CTA GAA GGG TAC GTC GTT AC 3′
parssbNcoI 5′ GGA GGA
    CC
    A TGG
GGC GCG GTG TTA ATA AAG TTA TCA TC 3′
parssbBglII 5′ TTA
    AGA TCT
CTA GAA AGG AAT GTC ATC GTC 3′
pinssbNcoI 5′ TTA
    CC
    A TGG
GGT TTA ACC GAA GCG TAA ACA AAG TAG 3′
pinssbHindIII 5′ TTA
    AAG CTT
CTA AAA AGG AAT ATC ATC ATC GAA ATC 3′

The boldface parts of the primers sequences are complementary to the nucleotide sequences of the ssb-like genes and the underlined parts are the recognition sites for restriction endonucleases.

Nowak et al.

Nowak et al. BMC Microbiology 2014 14:91   doi:10.1186/1471-2180-14-91

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