Table 4

Protein-protein interactions assayed by Bacterial adenylate cyclase two-hybrid system
Protein TpsA TpsB TpsC TppA
TpsA 418 (210–863)* 1746 (1582–1799) 113 (77–135) 71 (43–89)
TpsB 1593 (1467–1832) 1776 (1658–1988) 441 (341–560) 581 (322–714)
TpsC 172 (101–244) 688 (315–980) 1214 (861–1551) 80 (67–102)
TppA 429 (167–656) 691 (462–987) 156 (133–198) 83 (58–98)

*Estimated values are in units/mg dry weight bacteria. Values in parentheses are the highest and lowest scores for each based on three to four independent assays. The positive control zip-zip (T18 and T25 fragments of the leucine zipper of GCN4) was scored to 3429 (2938–4270). Negative controls and remaining protein interactions scored at maximum 220 (zip-tpsA) but usually less than 50. Values in bold are considered true protein-protein interactions.

Svanström et al.

Svanström et al. BMC Microbiology 2014 14:90   doi:10.1186/1471-2180-14-90

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