Quantitative expression of different lsc genes and constructs in dependence of lscB.lscBUpNA shows similar levels of expression as lscB while lscBUpA, which does not contain the first 48 bp of lscB ORF, has lower expression. lscA and lscAUpB were not seen to be expressed. lscA, lscBUpNA and lscBUpA were detected using lscA primers (1) while the rest using lscB primers (2). The data represent the mean relative expression of 3 replicates ± standard deviations. Data were normalized to the highest expression value of lscB, which was set to 100%.
Khandekar et al. BMC Microbiology 2014 14:79 doi:10.1186/1471-2180-14-79