ST6GAL1-specific siRNAs inhibited ST6Gal 1 expression and SAα2,6Gal synthesis. Respiratory epithelial cells were transfected with control or ST6GAL1 siRNAs (A) At 48 h post-transfection, ST6GAL1 mRNA expression levels were quantified. (B) Candidate siRNAs reduce ST6GAL1 expression in a dose-dependent manner. (C) FACS analysis showed a decrease in SAα2,6Gal expression on ST6GAL1 siRNA-treated cell surfaces. (D) Inhibition of ST6Gal Ι expression due to ST6GAL1 siRNAs. (E) We used ST6GAL1 siRNAs at various concentrations and they were not cytotoxic, except at 50 nM. aP < 0.05.
Wu et al. BMC Microbiology 2014 14:78 doi:10.1186/1471-2180-14-78