Expression of CCR5 ligands in liver and spleen tissues from infected mice. Wild type (WT) and CCR5−/− (KO) mice were infected intraperitoneally with T. gondii tachyzoites. Liver and spleen tissues were collected and their total RNA content was isolated at 0, 3 and 5 days post-infection (dpi). Expression of the target mRNA was determined and compared to expression levels at 0 dpi using quantitative PCR. Bars represent the average for each experimental group (0 dpi, n = 3; 3 dpi, n = 5; 5 dpi, n = 9). RH-GFP (GFP): parasites transfected with GFP alone; RH-OE (OE): parasites transfected with TgCyp18HA and GFP.
Ibrahim et al. BMC Microbiology 2014 14:76 doi:10.1186/1471-2180-14-76