Table 1

Bacterial strains and plasmids used in this study
Strains/plasmids Relevant characteristics* Source/reference
Strains
  S. suis
  05ZYH33 A highly virulent strain isolated from a dead patient with STSS Lab collection
  ΔvirB1-89K An isogenic virB1-89K mutant of strain 05ZYH33; Spcr [12]
  CΔvirB1-89K Complemented strain of ΔvirB1-89K; Spcr; Cmr [12]
  M. lysodeikticus
  ATCC4698 Suitable for substrate for the assay of lysozyme Sigma-Aldrich
  E. coli
  DH5α Cloning host for maintaining the recombinant plasmids Lab collection
  BL21(DE3) Expression host for exogenous protein production Lab collection
Plasmids
  pMD19-T Cloning vector; Ampr TaKaRa
  pET-21a(+) His-tag fusion expression vector; Ampr Novagen
  pET21a-CHAP A recombinant vector with the background of pET-21a(+), designed for expression of the CHAP domain of VirB1-89K; Ampr This work

*Ampr, ampicillin resistant; Cmr, chloramphenicol resistant; Spcr, spectinomycin resistant.

Zhong et al.

Zhong et al. BMC Microbiology 2014 14:73   doi:10.1186/1471-2180-14-73

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