Over-expression and purification of VirB1-89KCHAP. (A) SDS-PAGE analysis (12%) of the interest VirB1-89KCHAP protein expressed in E. coli. Lanes: 1, total crude extracts after IPTG induction; 2, sonicated supernatant of induced cells; 3, sonicated sediment of induced cells; 4, noninduced cells. Protein size marker is indicated on the left. (B) The purified VirB1-89KCHAP protein after Ni+ affinity chromatography and gel filtration.
Zhong et al. BMC Microbiology 2014 14:73 doi:10.1186/1471-2180-14-73