Table 1

β-galactosidase activities (units mg-1) for bacterial two-hybrid analysis of RbaW interactions with other proteins
Prey Bait
pT18c-RbaW pT18c pT18c-Zipa
pKNT25 RbaV 1440.0 ± 299.0 101.4 ± 53.7 NDb
RpoD 131.9 ± 18.6 165.0 ± 70.6 ND
RpoHI 212.7 ± 58.5 139.9 ± 32.2 ND
σ2637 310.7 ± 13.9 124.2 ± 22.9 ND
σ699 181.7 ± 54.3 201.7 ± 72.2 ND
Empty 147.0 ± 20.6 173.6 ± 23.7 ND
pKT25 RbaV 129.4 ± 15.9 115.8 ± 32.2 ND
RpoD 236.0 ± 60.8 132.4 ± 47.1 ND
RpoHI 161.0 ± 43.4 161.0 ± 6.6 ND
σ2637 220.5 ± 54.7 178.7 ± 28.3 ND
σ699 182.3 ± 63.4 199.1 ± 80.0 ND
Empty 130.4 ± 1.7 175.6 ± 9.1 ND
KT-Zipa ND ND 7338.9 ± 1300.0

aControl vector carrying fusions to leucine zipper peptide.

bNot determined.

Mercer and Lang

Mercer and Lang BMC Microbiology 2014 14:71   doi:10.1186/1471-2180-14-71

Open Data