Effects of rba mutations and in trans expression of rba genes on RcGTA gene transfer activity and protein levels. A. The ratio of gene transfer activity for each indicated strain relative to the parental strain, SB1003. The gene transfer activity was determined as an average relative to SB1003 in 3 replicate bioassays and the error bars represent the standard deviation. RcGTA production levels that differed significantly from the wild type were identified by analysis of variance (ANOVA) and are indicated by an asterisk (*; p < 0.05) or two asterisks (**; p < 0.1). B. Western blot detection of the RcGTA major capsid protein in the cells and culture supernatants of indicated strains. Blots were performed on all replicate gene transfer bioassay cultures (in A) and one representative set of blots is shown.
Mercer and Lang BMC Microbiology 2014 14:71 doi:10.1186/1471-2180-14-71