Table 2

Plasmid copy number determination for pZ7C and pZ7-184 in Z. mobilis NCIMB 11163, CU1 Rif2 and ATCC 29191 strains
Z. mobilis host strain and established shuttle vector Plasmid copy number
NCIMB 11163
pZ7C 1.8 ± 0.2
pZ7-184 1.2 ± 0.2
CU1 Rif2
pZ7C 1.7 ± 0.3
pZ7-184 2.8 ± 0.3
ATCC 29191
pZ7C 25.1 ± 1.4
pZ7-184 21.8 ± 1.6

Quantitative PCR (qPCR) was used to determine shuttle vector copy number determined using primers targeting the chloramphenicol acetyltransferase (cat) gene. Strains were cultivated in RM media containing 100 μg/ml chloramphenicol (Cm) at 30°C for 24 hours.

So et al.

So et al. BMC Microbiology 2014 14:68   doi:10.1186/1471-2180-14-68

Open Data