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Open Access Research article

pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis

Lok Yan So14, Wen-yang Chen1, Donnabella C Lacap-Bugler2, Myriam Seemann3 and Rory M Watt1*

  • * Corresponding author: Rory M Watt rmwatt@hku.hk

  • † Equal contributors

Author Affiliations

1 Oral Biosciences Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong

2 Oral Diagnosis and Polyclinics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong

3 Université de Strasbourg, CNRS UMR 7177, Institut Le Bel, 4 rue Blaise Pascal, CS 90032, Strasbourg 67081Cedex, France

4 Present Address: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong

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BMC Microbiology 2014, 14:68  doi:10.1186/1471-2180-14-68

Published: 15 March 2014

Additional files

Additional file 1:

Primers used in this study.

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Open Data

Additional file 2:

Restriction analysis of native plasmid DNA extracted from Z. mobilis NCIMB 11163.

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Open Data

Additional file 3:

Predicted positions of open reading frames and putative gene regulatory elements on plasmid pZMO7.

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Open Data

Additional file 4:

Stability of pZ7C shuttle vector in Z. mobilis NCIMB 11163, CU1 Rif2 and ATCC 29191 strains cultured in media with/without selection agent.

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Open Data

Additional file 5:

Quantitative-PCR determination of plasmid copy number for pZMO1A and pZMO7 in Z. mobilis NCIMB 11163 throughout the growth cycle.

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Additional file 6:

Affinity-purification of recombinant GST protein expressed from plasmid pZ7-GST established in E. coli BL21 (DE3), and Z. mobilis ATCC 29191 and CU1 Rif2.

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Additional file 7:

Growth curves for wild type and pZ7C-GST plasmid-transformed Z. mobilis strains NCIMB 11163, CU1 Rif2 and ATCC 29191.

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Additional file 8:

Expression of GST-fusion proteins from respective pZ7-GST plasmid constructs established in E. coli.

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Open Data

Additional file 9:

Western blot analysis of pZ7C-GST fusion protein expression levels in Z. mobilis ATCC 29191 and CU1 Rif2.

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Open Data