Quercetin reduced PRRSV infection in PAMs. (A) PAMs were treated with serial concentrations of Qct as indicated for 24 hours. Cell viability was measured with alamarBlue. Untreatment with quercetin served as control. Data are mean ± SD, n = 3, *p < 0.05 versus control (Ctrl). (B) PRRSV-infected PAMs were infected with PRRSV at an MOI of 0.1 for 1 hour and subsequently treated with Qct at the concentration as indicated. Mock-infected cells were untreated. The culture supernatants were collected at 24 h.p.i and viral titers were determined by calculating by CCID50. (C) Cells were harvested for Western blotting analysis. The relative protein level was quantified by measuring band intensities and normalized with respect to the amount of β-actin. Data are mean ± SD, n = 3. **p < 0.01, ***p < 0.001.
Gao et al. BMC Microbiology 2014 14:64 doi:10.1186/1471-2180-14-64