The knockdown of HSP70 reduced the level of viral dsRNA RI. MARC-145 cells were transfected with no siRNA (Ctrl), scramble siRNA (Scr), or different concentrations of siRNAs targetting HSP70 (siHSP70). After 24 hours, cells were mock infected or infected with PRRSV at an MOI of 0.1. Cells were fixed at 24 h.p.i and IFA was performed to detect viral dsRNA (red) with anti-dsRNA (J2) antibody. Nuclei were stained with Hoechst dye 33258 (blue). Bar, 200 μm.
Gao et al. BMC Microbiology 2014 14:64 doi:10.1186/1471-2180-14-64