Quercetin reduced PRRSV production. (A) MARC-145 cells were treated with serial concentrations of quercetin (Qct) as indicated for 24 hours. Cell viability was measured with alamarBlue. Untreatment with quercetin served as control. Data are mean ± SD, n = 3, *p < 0.05 versus control (Ctrl). (B) MARC-145 cells were incubated at 45°C for 20 minutes and then recultured at 37°C. Cells were harvested at different times as indicated for Western blotting analysis using anti-HSP70 and anti-β-actin antibodies. (C) MARC-145 cells were heated (HS) or not at 45°C for 20 minutes, and 8 hours later cells were inoculated with PRRSV at an MOI of 0.1 for 1 hour. Cells without heat shock treatment were subsequently cultured with medium containing DMSO, Qct (100 μM), or no chemical (Ctrl). The culture supernatants were collected at different times and viral titers were determined by calculating CCID50. Data are mean ± SD, n = 3.
Gao et al. BMC Microbiology 2014 14:64 doi:10.1186/1471-2180-14-64