PRRSV infection induced the HSP70 expression. (A) MARC-145 cells were infected with PRRSV at an MOI of 0.1 and harvested at different times as indicated. The total RNA was extracted to examine the HSP70 and viral N gene mRNA levels by quantitative RT-PCR. The levels of HSP70 and N mRNA were normalized with the level of GAPDH mRNA. (B) Cell extracts were analyzed by Western blotting using anti-HSP70, anti-N, and anti-β-actin antibodies. β-actin served as an internal reference. The levels of HSP70 and viral N protein were quantified by measuring band intensities and normalized with respect to the amount of β-actin. (C) MARC-145 cells were infected with PRRSV at different MOI as indicated. Cells were harvested at different time points for Western blotting analysis. Data are mean ± SD, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001.
Gao et al. BMC Microbiology 2014 14:64 doi:10.1186/1471-2180-14-64