Qualitative assessment of amylase activity by zymography. Zymography was performed to confirm that α-amylase activity at pH 9 and 25°C was optimal, in comparison with standards (A. oryzae and A. niger). M = molecular weight markers. A common band was detected at around 70 kDa for P. minima.
Zaferanloo et al. BMC Microbiology 2014 14:55 doi:10.1186/1471-2180-14-55