Biofilm inhibition by posaconazole and tobramycin. A. Effects of posaconazole alone and in combination with tobramycin against A. fumigatus monomicrobial and A. fumigatus-P. aeruginosa polymicrobial biofilms. B. Effects of tobramycin alone and in combination with posaconazole against P. aeruginosa monomicrobial and P. aeruginosa-A. fumigatus polymicrobial biofilms. Each experiment was performed two different times with the clinical isolates AF53470 and PA57402 using independently prepared conidial suspensions and bacterial cultures, and one time with the laboratory isolates AF36607 and PA27853. Both clinical and laboratory isolates provided similar results. The data were analyzed by one-way and two-way ANOVA with Bonferroni’s multiple comparison test where each set of data is compared with all the other sets of data as well as by paired two-tailed Student’s t-test using Graphpad Prism 5.0. The vertical bar on each data point denotes standard error of the mean for two independent experiments performed with the clinical isolates. Legends: AF, A. fumigatus monomicrobial biofilm; PA, P. aeruginosa monomicrobial biofilm; AF + PA and PA + AF, polymicrobial biofilm; PCZ, posaconazole; TOB, tobramycin.
Manavathu et al. BMC Microbiology 2014 14:53 doi:10.1186/1471-2180-14-53